New York State Route 427 (NY 427) is an east–west state highway in Chemung County, New York, in the United States. It extends for 11.5 miles (18.5 km) from its western terminus at an intersection with NY 14 in the town of Southport, south of the city of Elmira, to its eastern terminus at an interchange with NY 17 in the town of Chemung. Between those two towns, the highway passes through the town of Ashland and serves the village of Wellsburg.
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