West Virginia Route 527 is a north–south state highway located entirely in the Huntington, West Virginia area. The southern terminus of the route is at Interstate 64 exit 8 south of the city, where WV 527 becomes West Virginia Route 152 upon crossing the expressway. The northern terminus is on the West Virginia Department of Transportation-maintained Robert C. Byrd Bridge, better known as the Sixth Street Bridge, where the route becomes State Route 527 upon crossing into Ohio.
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