The Chalmers Institute is a historic building in Holly Springs, Mississippi, USA. Built in 1837, it was home to the University of Holly Springs, the oldest university in Mississippi, from 1838 to 1839. It was home to a short-lived Methodist medical and law school from 1839 to 1843. It reopened as the Chalmers Institute, a Presbyterian boys' school, from 1850 to 1878, when a yellow fever epidemic closed down the school.