Jean Hepburn, Lady Darnley, Mistress of Caithness, Lady Morham (died 1599) was a Scottish noblewoman and a member of the Border clan of Hepburn. Her brother was James Hepburn, Earl of Bothwell, the third husband of Mary, Queen of Scots. Jean's first husband was John Stewart, 1st Lord Darnley, an illegitimate half-brother of Queen Mary, which made Jean a double sister-in-law of the queen. Jean married three times.