Margaret of Parma (28 December 1522 – 18 January 1586) was Governor of the Netherlands from 1559 to 1567 and from 1578 to 1582. She was the illegitimate daughter of the then 22-year-old Holy Roman Emperor Charles V and Johanna Maria van der Gheynst. She was a Duchess of Florence and a Duchess of Parma and Piacenza by marriage.