Mary of Guise (French: Marie de Guise; 22 November 1515 – 11 June 1560) was Queen of Scots from 1538 to 1542 as the second wife of King James V. She was the mother of Mary, Queen of Scots, and served as Regent of Scotland in her daughter's name from 1554 to 1560. A native of Lorraine, she was a member of the powerful House of Guise, which played a prominent role in 16th-century French politics.