State Route 289 (SR 289) is a 1.637-mile-long (2.634 km) state highway in Humboldt County, in the northwestern part of the U.S. state of Nevada. The route serves the town of Winnemucca, and is concurrent with Interstate 80 Business (I-80 Bus.) for part of its length. The highway was formerly part of SR 1 and later U.S. Route 40 (US 40).