Night Off (1962 – after 1978) was an Irish-bred, British-trained Thoroughbred racehorse and broodmare best known for winning the classic 1000 Guineas in 1965. Despite running only twice in 1964 she was the top-rated filly in Britain after a win in the Cheveley Park Stakes. After recovering from illness to win the 1000 Guineas in April 1965 her form deteriorated, with a second place at Royal Ascot being her only notable performance in five subsequent races.