The Nizhyn Regiment (Ukrainian: Ніжинський полк) was one of ten territorial-administrative subdivisions of the Cossack Hetmanate. The regiment's capital was the city of Nizhyn, now in Chernihiv Oblast of central Ukraine. Other major cities of the regiment were Hlukhiv and Baturyn. The Nizhyn Regiment was founded in 1648, in 1653 it annexed the territory of the Novhorod-Siverskyi Regiment. Around that time the Regiment consisted of 10 sotnias.