State Route 329 (SR 329) is a state highway located in southeastern Ohio. The route is signed as a north–south route though the shape of the road is more of a backwards C. The southern terminus of SR 329 is in the Rome Township community of Guysville at US 50 and SR 32. After traveling north through the Athens County communities of Stewart and Amesville, the route briefly enters Morgan County before re-entering Athens County and ending at SR 13 in the village of Trimble.