René Bot (born 6 November 1978 in Velsen) is a former Dutch footballer. He normally played as a defender in a right back position, but could also play as a centre back. He started his career with Feyenoord but only played six matches in the first team. He played ten years for De Graafschap, where he was part of the first team for ten years. He ended his career with Eerste Divisie side AGOVV Apeldoorn. Due to a long period of injuries he only played eleven matches in two years.