Jesse Donald "Uncle Jimmy" Thompson (1848 – February 17, 1931) was an American old-time fiddle player. He is best remembered as the first performer to play on Nashville's Grand Ole Opry (then called the WSM Barn Dance), appearing with founder and host George D. Hay on the evening of November 28, 1925. The positive response generated by Thompson's performance would be an important influence on the show's creative direction in its formative years.