West Virginia Route 45 (WV 45) is a state highway in the U.S. state of West Virginia. The state highway runs 25.8 miles (41.5 km) from the Virginia state line near Glengary east to WV 480 and WV 230 in Shepherdstown. WV 45 connects the communities of Glengary and Arden in southwestern Berkeley County with the county seat of Martinsburg. The state highway also connects Shepherdstown in northern Jefferson County with Martinsburg, where the highway meets Interstate 81 (I-81), U.S.